Sperm whale insulin and pork insulin

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. As described in previous papers 1 , our experimental results, using the method of Moore and Stein 2 , have shown that the amino-acid composition of whale sei- insulin differs from that reported by Sanger et al.
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Structure of Sperm- and Sei-Whale Insulins and their Breakdown by Whale Pepsin

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Structure of Sperm- and Sei-Whale Insulins and their Breakdown by Whale Pepsin | Nature

Dysregulation of insulin or glucagon can result in loss of blood glucose control, characterized by hyperglycemia or hypoglycemia. To better understand the endocrine physiology of cetaceans, we cloned and characterized the insulin and glucagon genes from pygmy sperm whale Kogia breviceps. We obtained the complete coding sequences of the preproinsulin and preproglucagon genes, which encodes the preproinsulin protein of amino acid aa residues and encodes the preproglucagon protein of aa residues, respectively. Sequence comparison and phylogenetic analyses demonstrate that protein structures were similar to other mammalian orthologs. Immunohistochemistry and immunofluorescence staining using insulin, glucagon, and somatostatin antibodies allowed analysis of pygmy sperm whale islet distribution, architecture, and composition.
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